STR Genotyping: Precision Without the Complexity

STR Genotyping: Precision Without the Complexity

For labs working with repeat expansion models, STR genotyping is essential. It provides critical insights into repeat size and stability, especially in Huntington’s Disease research.

Traditional approaches can be complex, time-consuming, and difficult to scale, prompting the need for innovation.

This is where Transnetyx STR Genotyping comes in: it uniquely simplifies repeat analysis so you can generate dependable data without slowing your research.

What STR Genotyping Measures

STR genotyping determines the size of short tandem repeats within DNA. This includes CAG repeat sizing and the evaluation of somatic instability, both of which are key factors in understanding disease progression and the integrity of research models.

Accurate repeat measurements are not just data points. Without accurate repeat sizing, downstream decisions and study outcomes are at risk.

A Streamlined, Dual-Method Approach

Transnetyx STR Genotyping combines real-time PCR with fragment analysis to deliver both genotype and repeat size.

Real-time PCR uses fluorescent TaqMan assays to detect allele-specific sequences. Fragment analysis uses capillary electrophoresis to measure repeat length with high precision.

Together, these methods provide a complete and validated view of each sample.

Flexible Testing Options Built Around Your Study

Different research needs require different approaches. Transnetyx offers STR-only testing for repeat size and unique combined qPCR and STR testing for comprehensive genotype and repeat data. Reflex testing reduces cost by focusing STR sizing on relevant samples, offering unmatched flexibility.

For deeper analysis, CAG instability testing measures somatic variation using a standardized threshold and delivers detailed reporting, including raw data files and visual outputs.

Turnaround Time That Keeps Research Moving

Delays in repeat sizing can slow entire studies.

STR Genotyping results are typically delivered within three to five business days. CAG instability analysis is completed within ten business days.

Quicker access to results enables more confident decisions and reduces workflow bottlenecks.

Designed for Consistency and Scalability

Manual processes introduce variability. As sample volume increases, so does the risk of inconsistency.

Transnetyx provides built-in standardization that reduces variability and improves data consistency across samples and studies, with no added complexity for your team.

Getting Started Is Straightforward

To begin, submit your strain information for eligibility review. Transnetyx will confirm whether your strains qualify for testing. Current testing supports human and mouse models with fewer than 250 repeats.

After receiving approval, prepare your samples in accordance with the provided submission requirements and submit them with your completed order template.

Transnetyx will provide clear submission guidelines and standardized processes to help reduce errors and avoid delays.

The Bottom Line

STR genotyping is critical for understanding repeat biology, but it does not need to be complicated or resource-intensive.

With a streamlined workflow, flexible testing options, and reliable turnaround times, Transnetyx STR Genotyping delivers the precision you need without slowing your research.